RBS

Part:BBa_K2787035:Design

Designed by: Haoer Shi   Group: iGEM18_ShanghaiTech   (2018-10-09)


RFP with LVA tag under innate RBS, GFP with LVA tag under C9 orthogonal RBS with orthogonal 16S rRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 949
    Illegal NheI site found at 972
    Illegal NheI site found at 1879
    Illegal NheI site found at 1902
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1652


Design Notes

No


Source

This part was fully synthesized according to the sequence provided in A network of orthogonal ribosome·mRNA pairs(doi:10.1038/nchembio719) by Rackham, Oliver and Chin, Jason W.


References